Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization
Abstract This study was conducted to investigate the expression and enzymatic characteristics of recombinant Oryctolagus cuniculus chymosin (ROCC) expressed in Pichia pastoris. SDS-PAGE of the partially purified supernatant showed two distinct molecular bands of approximately 40 kDa and 45 kDa sizes corresponding to chymosin and partially glycosylated chymosin, respectively. The proteolysis assay showed that rabbit … Read more
Abstract Viral transduction of eukaryotic cell Recombinants is one possibility to generate recombinant proteins efficiently, and among all virus-based expression systems, the baculovirus/insect cell expression system (BEVS) is undoubtedly the best known and applied. This chapter describes the individual steps of the system process: maintenance of insect cell cultures, transfection of recombinant DNA into different insect … Read more
Abstract Homologous recombination (HR) is an important DNA repair pathway and is essential for cell survival. It plays an important role in the repair of replication-associated injuries and is functionally connected to replication. Transcription is another cellular process that has emerged to have a connection to human resources. Transcription enhances HR, which is a pervasive … Read more