Challenges and Advances in Genome Editing Technologies in Streptomyces.

The genome of Streptomyces encodes a high number of natural product (NP) biosynthetic gene clusters (BGCs). Most of these BGCs are not expressed or are poorly expressed (commonly called silent BGCs) under traditional laboratory experimental conditions. These NP BGCs represent an unexplored rich reservoir of natural compounds, which can be used to discover novel chemical compounds. To activate silent BGCs for NP discovery, two main strategies, including the induction of BGCs expression in native hosts and heterologous expression of BGCs in surrogate Streptomyces hosts, have been adopted, which normally requires genetic manipulation.

So far, various genome editing technologies have been developed, which has markedly facilitated the activation of BGCs and NP overproduction in their native hosts, as well as in heterologousStreptomyces hosts. In this review, we summarize the challenges and recent advances in genome editing tools for Streptomyces genetic manipulation with a focus on editing tools based on clustered regularly interspaced short palindrome repeat (CRISPR)/CRISPR-associated protein (Cas) systems. Additionally, we discuss the future research focus, especially the development of endogenous CRISPR/Cas-based genome editing technologies in Streptomyces

LRG Blocking Peptide

20-abx064040
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.

99445-20 DCT 20 X 150MM

99445-20 250/pk
EUR 119
Description: Disposable Culture Tubes; DCT's, CGW

Polyclonal LRG1 / LRG Antibody

APR17232G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LRG1 / LRG . This antibody is tested and proven to work in the following applications:

20(S)-NotoginsenosideR2

N2028-20 20 mg
EUR 630
Description: Extracted from Pseudo-ginseng;Store the product in sealed, cool and dry condition

FGF-20, human recombinant

4589-20
EUR 277

20(R)Ginsenoside?Rh1

N2192-20 20 mg
EUR 514
Description: Extracted from Panax ginseng C. A. Mey. dried roots;Store the product in sealed, cool and dry condition

20(R)Ginsenoside Rg2

N2195-20 20 mg
EUR 224
Description: Extracted from Panax ginseng C. A. Mey. dried roots;Store the product in sealed, cool and dry condition

20(R)Ginsenoside Rg3

N2198-20 20 mg
EUR 514
Description: Extracted from Panax ginseng C.A.Mey. roots;Store the product in sealed, cool and dry condition

Polyclonal IRGM / LRG-47 Antibody (Internal)

APR08028G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IRGM / LRG-47 (Internal). This antibody is tested and proven to work in the following applications:

20UL FILTER TIPS FOR P-20

TF-20 1000/pk
EUR 418
Description: Filter Tip; Filter Tips Pipette - Axygen

AXYGEN® GEL TRAY 20 X 20 CM FOR USE WITH 20 CM GEL BOX, UV TRANSPARENT

HGB20-20-GT 1/pk
EUR 97
Description: Lab Equipment; Axygen Branded EQ

DiagNano Silica-Coated Gold Nanoparticles, 20 nm

CG-20 10mL
EUR 719

AXYGEN® HORIZONTAL GEL BOX, 20 CM

HGB-20 1/pk
EUR 541
Description: Lab Equipment; Axygen Branded EQ

99447 DSSCT 20 X 125 W/ MARKING SPOT

99447-20 250/pk
EUR 211
Description: Disposable Screw Cap Culture Tubes; DSCCT's, Lab Stock

DiagNano Gold Nanoparticle Passive Conjugation Kit, 20 nm

GPK-20 1 kit
EUR 715

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 20 nm

PG-20 10mL
EUR 719

99449 DSSCT 20 X 125MM W/O MARKING SPOT

99449-20 250/pk
EUR 228
Description: Disposable Screw Cap Culture Tubes; DSCCT's, Lab Stock

Single channel mini-pipette (130 mm) 20 ul, autoclavable

SCMP-20 1
EUR 92

Exosome binding buffer for ELISAs (20 ml)

EXOEL-BB-20 20 ml
EUR 219
  • Category: Exosomes

TumorExoRNA? Tumor-derived exosome immunocapture and RNA extraction kit (20 reactions)

K1221-20
EUR 1219

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)

M1030-20
EUR 1289

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 20 reactions)

M1031-20
EUR 1289

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)

M1032-20
EUR 1349

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 20 reactions)

M1033-20
EUR 1349

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions)

M1034-20
EUR 1246

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 20 reactions)

M1035-20
EUR 1246

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 20 reactions)

M1036-20
EUR 1289

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 20 reactions)

M1037-20
EUR 1289

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 20 reactions)

M1039-20
EUR 1289

ExoDNAPS? Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 20 reactions)

K1230-20
EUR 718

ExoDNAUC? Circulating and Exosome-associated DNA Extraction Kit (Urine/Cell Media, 20 reactions)

K1231-20
EUR 718

20 bp random library with flanking sequence; DNA aptamer

DAL-N-20 100 ug
EUR 286

DiagNano Silica-Coated Gold Nanoparticles, 20 nm, 100 OD

CG-20-100 0.25mL
EUR 772

DiagNano Gold Nanoparticle Medium Covalent Conjugation Kit, 20 nm

GCK-M-20 1 kit
EUR 757

DiagNano Fluorophore Labeled Gold Nanoparticles, 20 nm, Cellular Uptake

GFL-20-CU 1mL
EUR 1365

Custom Anti-Peptide antibodies (peptide synthesis up to 20-aa, conjugation, 2 rabbits, ELISA)

ABPEP-20 1
EUR 1626

TotalExoRNA? Overall exosome immunocapture and RNA extraction kit (Biological Fluids/Cell Media, 20 reactions)

K1220-20
EUR 1219

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 20 nm, 100 OD

PG-20-100 0.25mL
EUR 918

Oligo (dT)20 Coated Gold Particles

OdT(20)AuP-002-1 1 mL
EUR 385
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Oligo (dT)20 Coated Gold Particles

OdT(20)AuP-002-5 5 mL
EUR 1045
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Rabbit Anti-B. pertussis Lipopolysaccharide (LPS) IgG ELISA kit, 96 tests, Quantitative

960-420-LRG 1 Kit
EUR 834

Polyoxyethylene-20 (TWEEN 20)

TB0560 500ml
EUR 66.64
  • Product category: Biochemicals/Detergents/Surfactants

AXYGEN® AXYPET® PRO 2-20 ΜL SINGLE-CHANNEL PIPETTOR, AUTOCLAVABLE

AP-20-P 1/pk
EUR 202
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

20UL FILTER TIPS FOR P-20, RACKED & PRE-STERILIZED

TF-20-R-S 960/pk
EUR 258
Description: Filter Tip; Filter Tips Pipette - Axygen

20-Hydroxy-20-epi-tingenone

TBZ1426 unit Ask for price

IL-20, Interleukin-20, human

RC212-31 2ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Cytokines

AXYGEN® 20-WELL COMB FOR USE WITH 15 CM GEL BOX, 0.75 MM THICKNESS

HGB15-20-075 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 20-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.0 MM THICKNESS

HGB15-20-1 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 20-WELL COMB FOR USE WITH 15 CM GEL BOX, 1.5 MM THICKNESS

HGB15-20-15 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® 20-WELL COMB FOR USE WITH 15 CM GEL BOX, 2.0 MM THICKNESS

HGB15-20-2 1/pk
EUR 60
Description: Lab Equipment; Axygen Branded EQ

Human Cytokeratin-20 (CK-20) Antibody

31313-05111 150 ug
EUR 261

TWEEN 20, Polyoxyethylene-20-Sorbitan Monolaurate

CH063 1L (2 x 500ml)
EUR 132

TWEEN 20, Polyoxyethylene-20-Sorbitan Monolaurate

CH064 4.0 L
EUR 177

Recombinant Human Interleukin-20/IL-20

C147-10ug 10ug
EUR 202
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-20/IL-20

C147-1mg 1mg
EUR 2283
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-20/IL-20

C147-500ug 500ug
EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-20/IL-20

C147-50ug 50ug
EUR 496
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Gel Tray 20 X 20 Cm

LE1000GT-06 Ea
EUR 188

Custom Anti-Peptide antibodies, 2 peptides (upto 20-aa; synthesis, 2 conjugation, 2 rabbits, 2 ELISA)

ABPEP-20-2 1
EUR 2356

Tween 20

22002 50ML
EUR 113
Description: Minimum order quantity: 1 unit of 50ML

Tween-20

CT371 5 ml
EUR 49

Tween 20

abx082486-100ml 100 ml
EUR 175
  • Shipped within 5-10 working days.

Tween 20

abx082594-100ml 100 ml
EUR 189
  • Shipped within 5-10 working days.

20-HETE

HY-15598 2mg
EUR 243

20-Deoxyingenol

HY-N0866 10mg
EUR 298

Tween 20

GD9856-100ML 100 ml
EUR 42

Tween 20

GD9856-1L 1 l
EUR 70

Tween 20

GD9856-250ML 250 ml
EUR 49

Tween 20

GD9856-500ML 500 ml
EUR 54

Tween 20

GD9856-5L 5 l
EUR 150

Tween 20

TWN500 500 ml
EUR 79

Tween 20

TWN999 1000 ml
EUR 92

Raddeanoside 20

TB0744-0010 5mg
EUR 313

Sarkosyl 20%

TG4065 5ml
EUR 168

20-Deoxyingenol

TRQ0095 unit Ask for price

Human cytokeratin 20,CK-20 ELISA Kit

201-12-1719 96 tests
EUR 440
  • This cytokeratin 20 ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Interleukin 20,IL-20 ELISA KIT

201-12-2164 96 tests
EUR 440
  • This Interleukin 20 ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

[Cys(Acm)20, 31]-EGF (20-31)

5-00069 4 x 5mg Ask for price

Human Interleukin 20 (IL-20) CLIA Kit

abx195883-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

IL-20/ Rat IL- 20 ELISA Kit

ELA-E2058r 96 Tests
EUR 886

Human IL-20(Interleukin 20) ELISA Kit

EH0190 96T
EUR 524.1
  • Detection range: 62.5-4000 pg/ml
  • Uniprot ID: Q9NYY1
  • Alias: IL-20/IL20/ZCYTO10/IL10D
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 37.5pg/ml

Human Interleukin 20(IL-20)ELISA Kit

CSB-E15015h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 20 (IL-20) in samples from serum, plasma, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Interleukin 20(IL-20)ELISA Kit

1-CSB-E15015h
  • EUR 678.00
  • EUR 4644.00
  • EUR 2467.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 20(IL-20) in samples from serum, plasma, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human cytokeratin 20,CK-20 ELISA Kit

CN-04600H1 96T
EUR 464

Human cytokeratin 20,CK-20 ELISA Kit

CN-04600H2 48T
EUR 313

Chromosome 20 Open Reading Frame 20 Protein

20-abx262479
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Rat IL-20(Interleukin 20) ELISA Kit

ER1590 96T
EUR 524.1
  • Detection range: 31.2-2000 pg/ml
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 7.8pg/ml

Human cytokeratin 20(CK-20)ELISA Kit

GA-E1735HM-48T 48T
EUR 289

Human cytokeratin 20(CK-20)ELISA Kit

GA-E1735HM-96T 96T
EUR 466

IL-20 Interleukin-20 Mouse Recombinant Protein

PROTQ9JKV9 Regular: 10ug
EUR 317
Description: Interleukin-20 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a total molecular mass of 17,584 Dalton. The IL-20 is purified by proprietary chromatographic techniques.

IL-20 Interleukin-20 Human Recombinant Protein

PROTQ9NYY1 Regular: 10ug
EUR 317
Description: Interleukin-20 Human Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 2 x 153 amino acids and having a total molecular mass of 35,212 Dalton. ;The IL-20 is purified by proprietary chromatographic techniques.

Human cytokeratin 20(CK-20)ELISA Kit

QY-E00973 96T
EUR 361

Human Interleukin 20(IL-20)ELISA Kit

QY-E04295 96T
EUR 361

Rat Interleukin 20(IL-20)ELISA Kit

QY-E10616 96T
EUR 361

Mouse Interleukin 20(IL-20)ELISA Kit

QY-E20848 96T
EUR 361

20UL "MAXYMUM RECOVERY" FILTER TIPS FOR P-20, RACKED, PRE-STERILIZED

TF-20-L-R-S 960/pk
EUR 276
Description: Filter Tip; Maximum Recovery Filter Tips - Axygen

Human Cytokeratin-20 (CK-20) Antibody (Biotin Conjugate)

31313-05121 150 ug
EUR 369

CENTRIFUGE, STYROFOAM TUBE RACK, 50ML,NS,20/20

4365 20/pk
EUR 50
Description: Centrifuge Tubes; Corning Centrifuge Tubes
.