UTF1 is a transcriptional coactivator which has lately been remoted and discovered to be expressed primarily in pluripotent embryonic stem (ES) cells (A. Okuda, A. Fukushima, M. Nishimoto, et al., EMBO J. 17:2019-2032, 1998).
To acquire perception into the regulatory community of gene expression in ES cells, we’ve got characterised the regulatory parts governing UTF1 gene expression.
The outcomes point out that the UTF1 gene is one of the goal genes of an embryonic octamer binding transcription issue, Oct-3/4. UTF1 expression is, like the FGF-4 gene, regulated by the synergistic motion of Oct-3/4 and one other embryonic issue, Sox-2, implying that the requirement for Sox-2 by Oct-3/Four will not be restricted to the FGF-Four enhancer however is fairly a common mechanism of activation for Oct-3/4.
Our biochemical analyses, nevertheless, additionally reveal one distinct distinction between these two regulatory parts: not like the FGF-Four enhancer, the UTF1 regulatory element can, by its one-base distinction from the canonical octamer-binding sequence, selectively recruit the complex comprising Oct-3/4 and Sox-2 and preclude the binding of the transcriptionally inactive complex containing Oct-1 or Oct-6. Furthermore, our analyses reveal that these properties are dictated by the distinctive capability of the Oct-3/Four POU-homeodomain that acknowledges a variant of the Octamer motif in the UTF1 regulatory element.
The main chlorophyll a/b binding protein of petunia is composed of a number of polypeptides encoded by a quantity of distinct nuclear genes.
Five distinct cDNA clones for the main chlorophyll a/b binding protein (Cab) of the petunia thylakoid membrane light-harvesting complex have been characterised. The nucleotide sequences of the polypeptide coding areas are comparable between clones; nevertheless, the 3′-untranslated areas are divergent.
Analysis of petunia nuclear DNA via genomic hybridizations and characterization of cloned nuclear fragments signifies there are no less than sixteen genes for the main Cab protein. These genes might be categorised into no less than 5 small multigene households on the foundation of their homology to the totally different cDNA clones.
Two households include solely two genes every and in each instances the genes are intently linked in the genome. In one case, one gene is in an inverted orientation with respect to the different such that the 5′ ends are adjoining; in the different case, the two genes are in a tandem array.
Both genes from one of these households are transcribed in inexperienced leaves. The genes belonging to separate nuclear gene households encode totally different polypeptide elements of the main Cab protein.